·  1 Identification of substance:

• Product details:  Trade name: Fluorescein Free Acid  
• Manufacturer/Supplier:
  NILE CHEMICALS
  Web Site: www.nilechemicals.com

·    2 Composition/Data on components:

Chemical characterization:
Description: (CAS#)                                        
Fluorescein sodium salt (CAS# 2321-07-5), 100%

·    3 Hazards identification

• Hazard description: Xn Harmful
• Information pertaining to particular dangers for man and environment
  R 20/22 Harmful by inhalation and if swallowed.
  R 36/38 Irritating to eyes and skin.

·    4 First aid measures

• After inhalation
  Supply fresh air. If required, provide artificial respiration. Keep patient warm.
  After skin contact
  Immediately wash with water and soap and rinse thoroughly.
  After eye contact
  Rinse opened eye for several minutes under running water. Then consult a doctor.
• After swallowing Seek immediate medical advice.

·    5 Fire fighting measures

• Suitable extinguishing agents
  Carbon dioxide, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam.
• Special hazards caused by the material, its products of combustion or
  resulting gases:
  In case of fire, the following can be released:
  Carbon monoxide and carbon dioxide,Metal oxide fume
• Protective equipment:
  Wear self-contained respirator. Wear fully protective impervious suit.

·    6 Accidental release measures

• Person-related safety precautions:
  Wear protective equipment. Keep unprotected persons away.
  Ensure adequate ventilation
• Measures for environmental protection:
  Do not allow material to be released to the environment without proper governmental permits.
• Measures for cleaning/collecting:
  Dispose contaminated material as waste according to item 13.
  Ensure adequate ventilation.
• Additional information:
  See Section 7 for information on safe handling
  See Section 8 for information on personal protection equipment.
  See Section 13 for disposal information.

·    7 Handling and storage

• Handling
• Information for safe handling:
  Keep container tightly sealed.Store in cool, dry place in tightly closed containers. Ensure good ventilation at the workplace.
• Information about protection against explosions and fires:
  No special measures required.
• Storage
• Requirements to be met by storerooms and receptacles:
  No special requirements.
• Information about storage in one common storage facility:
  Store away from oxidizing agents.
• Further information about storage conditions:
  Keep container tightly sealed.
  Store in cool, dry conditions in well sealed containers.

·    8 Exposure controls and personal protection

• Additional information about design of technical systems:
  Properly operating chemical fume hood designed for hazardous chemicals and having an average face velocity of at least 100 feet per minute.
  
  Components with limit values that require monitoring at the workplace:
  Not required.
• Additional information: No data
• Personal protective equipment
• General protective and hygienic measures
  The usual precautionary measures for handling chemicals should be followed.
  Keep away from foodstuffs, beverages and feed.
  Remove all soiled and contaminated clothing immediately.
  Wash hands before breaks and at the end of work.
  Avoid contact with the eyes and skin.
• Breathing equipment:
  Use suitable respirator when high concentrations are present.
• Protection of hands: Impervious gloves
• Eye protection:
  Safety glasses :Tightly sealed goggles :Full face protection
• Body protection: Protective work clothing.

·    9 Physical and chemical properties:

• General Information
• Form: Powder
• Color: Dark orange powder
• Odor: Not determined
•                                      Value/Range  Unit    Method      
• Change in condition
• Melting point/Melting range:       Not determined
• Boiling point/Boiling range:       Not determined
• Sublimation temperature / start:   Not determined
• Flash point:                       Not applicable
• Ignition temperature:              Not determined
• Decomposition temperature:         Not determined
• Danger of explosion:
  Product does not present an explosion hazard.
• Explosion limits:
• Lower:                             Not determined
• Upper:                             Not determined
• Vapor pressure:                    Not determined
• Density:                           Not determined
• Solubility in / Miscibility with
• Water:                             Not determined

·   10 Stability and reactivity

• Thermal decomposition / conditions to be avoided:
  Decomposition will not occur if used and stored according to specifications.
• Materials to be avoided: Oxidizing agents
• Dangerous reactions No dangerous reactions known
• Dangerous products of decomposition:Carbon monoxide and carbon dioxide: Metal oxide fume

·   11 Toxicological information

• Acute toxicity:
  LD/LC50 values that are relevant for classification:                   
  Oral: LD50: 4738 mg/kg (mus)
  LD50: 6721 mg/kg (rat)
• Primary irritant effect:
• on the skin: Irritant to skin and mucous membranes.
• on the eye: Irritating effect.
• Sensitization: No sensitizing effects known.
• Other information (about experimental toxicology):
  Tumorigenic effects have been observed on tests with laboratory animals.
  Mutagenic effects have been observed on tests with laboratory animals.
  Mutagenic effects have been observed on tests with bacteria.
  Reproductive effects have been observed on tests with laboratory animals.
• Subacute to chronic toxicity:
  Ingestion of fluorescein disodium salt can cause nausea, ataxia and difficult or labored respiration.
• Additional toxicological information:
  To the best of our knowledge the acute and chronic toxicity of this substance is not fully known.
  The Registry of Toxic Effects of Chemical Substances (RTECS) contains tumorigenic and/or carcinogenic and/or neoplastic data for components in this product.
  No classification data on carcinogenic properties of this material is available from the EPA, IARC, NTP, OSHA or ACGIH.

·   12 Ecological information:

• General notes:
  Do not allow material to be released to the environment without proper governmental permits.

·   13 Disposal considerations

• Product:
• Recommendation
  Consult state, local or national regulations to ensure proper disposal.
• Uncleaned packagings:
• Recommendation:
  Disposal must be made according to official regulations.

·   14 Transport information

Not a hazardous material for transportation.

• DOT regulations:
• Hazard class:             None
• Land transport ADR/RID  (cross-border)
• ADR/RID class:                None
• Maritime transport IMDG:
• IMDG Class:               None
• Air transport ICAO-TI and IATA-DGR:
• ICAO/IATA Class:          None
• Transport/Additional information:
  Not dangerous according to the above specifications.

·   15 Regulations

• Product related hazard informations:
• Hazard symbols: Xn Harmful
• Risk phrases:
  20/22 Harmful by inhalation and if swallowed.
  36/38 Irritating to eyes and skin.
• Safety phrases:
  9     Keep container in a well-ventilated place.
  26    In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  36/37 Wear suitable protective clothing and gloves.
  60    This material and its container must be disposed of as hazardous waste.
• National regulations
  All components of this product are listed in the U.S. Environmental Protection Agency Toxic Substances Control Act Chemical Substance Inventory. 

·   16 Other information:

• Employers should use this information only as a supplement to other information gathered by them, and should make independent judgement of suitability of this information to ensure proper use and protect the health and safety of employees.  This information is furnished without warranty, and any use of the product not in conformance with this Material Safety Data Sheet, or in combination with any other product or process, is the responsibility of the user.